In situ Detection of Nucleid Acids
The project “In situ Detection of Nucleic Acids”, funded by the City of Vienna, aims to establish in situ hybridization methods for the detection of cellular RNA and DNA molecules, in particular the novel Hybridization Chain Reaction (HCR) method, and advanced competences in data processing and data analysis at the University of Applied Sciences Technikum Wien. The acquired knowledge is incorporated into the design and adaption of teaching materials in bachelor and master programs with a focus on state-of-the-art cytological methods, microscopic techniques, data processing with AI, deep clearing algorithms, as well as data quantification and classification. The established cytological protocols (including HCR) are processed in an online knowledge base and include researched marker genes for cell tissue in the field of tissue engineering as well as marker genes for toxicological analyses. The aquired competences in the fields of image processing, data quantification and classification are prepared for research at the FHTW in an application-oriented way. The state-of-the-art in situ hybridization methods can be used to study factors influencing cell systems, cell tissues and model organisms and is thus particularly relevant for the FHTW research field Tissue Engineering & Molecular Life Sciences Technology, as well as the bachelor’s degree program “Biomedical Engineering” and the master’s degree programs “Tissue Engineering and Regenerative Medicine” and “Ecotoxicology & Environmental Management”. The project team supports the infrastructure and competencies at the FHTW. To achieve this, bridge courses for standardized training in the use of microscopes and their operating software are developed and implemented, as well as personal training on the microscopic equipment for staff and student assistants of the FHTW. The project promotes the training of specialists for the city of Vienna by employing student assistants who work on the establishment of state-of-the-art cytological methods and protocols. The organization of school workshops for Viennese students is intended to arouse the interest of the younger generations in scientific observations. Furthermore, an annual picture competition “Wall of FHame” is organized by the project team.
